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Mechanism of T7 Primase / Helicase

From : SciAna film works www.scianafilms.com
Категория: Education
Время: 00:01:25.500
Теги: anim
 

DNA Replication Process

www.FreeScienceLectures.com We travel inside nucleus to see how the DNA replicates. When DNA replicates its strands are separated by enzine helicase. Single-stranded DNA binding proteines keep the strands from (...?). One DNA strand encodes the leading strand using DNA Polymerase III. Just watch to see what is going on. --- It's Never too Late to Study www.FreeScienceLectures.com --- Notice This video is copyright by its respectful owners. The website address on the video does not mean anything. ---
Категория: Howto & Style
Время: 00:01:29.250
Теги: dna replication enzine helicase strand polymerase encode okazaki fragments rna primase primer ligase
 

aging of primase

the aging of CoV primase
Категория: Science & Technology
Время: 00:00:30.750
Теги: protein structure movie
 

DNA Replication Cartoon

Our second cartoon made for our AP Biology class. Comments would be appreciated! We realize that some of this is hard to understand, so here is the script of the second half: "Helicases travel to the origin of replication to unwind the DNA, forming a replication fork. Single strand binding proteins hold the two template strands apart. On the leading strand, the enzyme primase attaches to the DNA to synthesize and add an RNA primer. Next, DNA polymerase III adds DNA nucleotides to the RNA primer, but only in the 5 prime to 3 prime direction. On the leading strand, nucleotides can be added continuously. The process is different for the lagging strand. First on the lagging strand, primase removes the single strand binding proteins. Then, it forms an RNA primer. DNA polymerase III adds nucleotides to the 3 prime end of the RNA primer. These short segments are called okazaki fragments. As a finishing touch, an enzyme called DNA ligase bonds the okazaki fragments together. This completes the lagging strand."
Категория: Film & Animation
Время: 00:03:15.750
Теги: biology cartoon science dna replication animation
 

DNA Replication, Recombination, Repair III

This course is part of a series taught by Kevin Ahern at Oregon State University on General Biochemistry. For more information about online courses go to ecampus.oregonstate.edu for the rest of the courses see www.youtube.com 1. Initiation of replication in E. coli occurs at a specific site on the E. coli genomic DNA, known as OriC, in the cell's circular chromosome. The OriC site contains three repeats of an AT rich sequence near some sequences bound by the DNA A protein. 2. Replication initiation begins with binding of the several copies of the DNA A protein to the OriC site. Bending and wrapping of the DNA around DNA A proteins causes the AT-rich sequences noted above to become single-stranded. 3. Next, the DNA BC complex binds the DNA B protein (helicase) to each of the single strands in opposite orientations. The DNA C protein is released in the process. Next, SSB and primase bind the exposed single-stranded regions and cause DNA A protein to be released. The primases begin synthesizing RNA primers (remember - 5' to 3' RNA synthesis only also) in opposite directions on each strand. The primases DO NOT require a pre-existing primer to function. 4. Note that replication is bi-directional - two replication forks pointed in opposite directions from the origin. They meet later at a termination site on the other side of the genomic DNA. 5. Eukaryotic DNA replication is coordinated tightly with the cell cycle. Checkpoints during the cell cycle ensure that progression ...
Категория: Education
Время: 00:38:12
Теги: oregon state university osu ecampus online lecture general biochemistry chemistry science biology online lecture course class
 

DNA Replication

Paul Andersen explains how DNA replication ensures that each cell formed during the cell cycle has an exact copy of the DNA. He describes the Meselson-Stahl experiment and how it showed that DNA copies itself through a semi-conservative process. He then explains how multiple enzymes, like DNA polymerase, helicase, primase, ligase, and single strand binding proteins copy DNA. He also differentiates between the leading and the lagging strand. He explains how DNA is anti-parallel in nature and how eukaryotic cells have multiple origins of replication.
Категория: Education
Время: 00:07:37.500
Теги: dna replication semiconservative DNA polymerase 5' 3' helicase leading strand lagging strand primase ligase origin of replication
 

DNA Structure and Replication: CrashCourse Biology #10

Hank introduces us to that wondrous molecule deoxyribonucleic acid - also known as DNA - and explains how it replicates itself in our cells. Like CrashCourse on Facebook: www.facebook.com Follow CrashCourse on Twitter: www.twitter.com References for this episode can be found in the Google document here: dft.ba 1:41 link to Biological Molecules www.youtube.com Table of Contents: 1) Nucleic Acids 1:30 2) DNA -A) Polymers 1:53 -B) Three Ingredients 2:12 -C) Base Pairs 3:45 -D) Base Sequences 4:13 3) Pop Quiz 5:07 4) RNA 5:36 -A) Three Differences from DNA 5:43 5) Biolography 6:16 6) Replication 8:49 -A) Helicase and Unzipping 9:22 -B) Leading Strand 9:38 -C) DNA Polymerase 10:08 -D) RNA Primase 10:24 -E) Lagging Strand 10:46 -F) Okazaki Fragments 11:07 -F) DNA Ligase 11:47 DNA, deoxyribonucleic acid, chromosome, nucleic acid, ribonucleic acid, RNA, polymer, nucleotide, double helix, nucleotide base, base pair, base sequence, friedrich miescher, rosalind franklin, replication, helicase, leading strand, lagging strand, rna primase, dna polymerase, okazaki fragment
Категория: Education
Время: 00:09:44.250
Теги: DNA deoxyribonucleic acid chromosome nucleic acid ribonucleic acid RNA polymer nucleotide double helix nucleotide base base pair base sequence friedrich miescher rosalind franklin replication helicase leading strand lagging strand rna primase dna polymerase okazaki fragment
 

Bite-Sized Biochemistry #43 - DNA Replication, Repair, Recombination III

(02/14/11) Lecture by Kevin Ahern of Oregon State University discussing Biochemistry Basics in BB 451. See the full course at oregonstate.edu This course can be taken for credit (wherever you live) via OSU's ecampus. For details, see ecampus.oregonstate.edu Download Metabolic Melodies at www.davincipress.com Related courses include BB 350 - oregonstate.edu BB 450 - oregonstate.edu BB 100 - oregonstate.edu DNA Replication/Repair/Recombination III 1. Initiation of replication in E. coli occurs at a specific site on the E. coli genomic DNA, known as OriC, in the cell's circular chromosome. The OriC site contains three repeats of an AT rich sequence near some sequences bound by the DNA A protein. 2. Replication initiation begins with binding of the several copies of the DNA A protein to the OriC site. Bending and wrapping of the DNA around DNA A proteins causes the AT-rich sequences noted above to become single-stranded. 3. Next, the DNA BC complex binds the DNA B protein (helicase) to each of the single strands in opposite orientations. The DNA C protein is released in the process (I said this backward in class). Next, SSB and primase bind the exposed single-stranded regions and cause DNA A protein to be released. The primases begin synthesizing RNA primers (remember - 5' to 3' RNA synthesis only also) in opposite directions on each strand. The primases DO NOT require a pre-existing primer to function. 4. Note that replication is bi-directional - two replication forks ...
Категория: Education
Время: 00:36:18
Теги: DNA replication repair telomere telomerase aging length initiation reverse transcriptase RNA template eukaryotic linear chromosome cancer fetal cells damage proofreading mismatch nucleotide excision base muts mutl muth thymine dimers UV light cross-link covalent Kevin Ahern biochemistry molecular biology basics fundamentals simple easy clear health Lesson Teachers Free University Tutorial Learn Tube Learning Tutorials Resource science medicine medical
 

#16 BB 350 DNA Replication I - Kevin Ahern's Biochemistry Online

Kevin Ahern of Oregon State University lecturing to his BB 350 class. See the full course at oregonstate.edu This course can be taken for credit (wherever you live) via OSU's ecampus. For details, see ecampus.oregonstate.edu Topics covered include DNA replication polymerase 5' 3' primase SSB helicase gyrase topoisomerase proofreading exonuclease primer RNA polymerase excision repair recombination AZT beta clamp PCNA replication fork thymine dimers 8 oxoguanine
Категория: Education
Время: 00:38:51
Теги: DNA replication polymerase 5' 3' primase SSB helicase gyrase topoisomerase proofreading exonuclease primer RNA excision repair recombination AZT beta clamp PCNA fork thymine dimers oxoguanine biochemistry Kevin Ahern lecture science scientific class online education simple easy understand chemistry molecular biology video Educational
 

DNA Replication Song (Take a bow)

I made this video in a rush 4 bio proj. So....hehhe my voice was a bit funny. Sr bout dat but anyway, hope u guys enjoy my video ^.^ Lyircs: Take a bow How about replication of DNA. Yeah, really interesting. Oh oh, yeah, yeah, yeah, yeah. DNA, DNA, everyone has DNA Now it's time to replicate. This replication process goes on and on. A pairs with T and G pairs with C. It works cause the code complementary. Helicase Helicase the first enzyme to replicate. It unzips the double strands. For next step to take its stand. That was quite a show, very entertaining. And its not over, go on and take next step. Primase Primase the second enzyme (to join the club). Making short strand of RNA. The bases pair up just like they did before. But T is replaced by U (What else is on?). DNA polymerase number one. Just wants to have some fun. So it completes the leading strand. Going from the 5 prime to the 3 prime of the strands direction. That was quite a show, very entertaining. And its not over, go on and take next step. DNA polymerase number two. Has its own job to do. Just replace the RNA With usable DNA (Lets clap the hands!!!). But the replication isnt done. We need one more enzyme called Ligase. It comes to complete all the gap. That was quite a show, very entertaining, but its over now. Go on and take a bow.\ It will start again!!!
Категория: Education
Время: 00:02:59.250
Теги: DNA replication song
 
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